Restriction Enzymes
New England Biolabs, Inc. XmaI – 500 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
ABclonal Technology SRF Rabbit pAb
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
New England Biolabs, Inc. SspI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. BbsI HF - 1000 units
A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind HF restriction enzymes are fully active under a broader range of conditions minimizing off-target products while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
MilliporeSigma GSK-3BETA RECOM ENZYME
GSK3 beta active Serine/Threonine Phosphorylation 5mcg 125mcg 250mcg Protein Phosphorylation Assay: 5-40ng PURITY:>95% by SDS-PAGE STABILITY: 6 months at - 20degreeC PROPERTIES: Specific activity guaranteed between 932 and 1269U/mg
New England Biolabs, Inc. XhoI – 5000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. AflII – 2000 units
A restriction endonuclease that recognizes the sequence C^TTAA_G.
New England Biolabs, Inc. PmeI – 500 units
A restriction endonuclease that recognizes the sequence GTTT_^AAAC.
New England Biolabs, Inc. PaqCI - 200 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. ScaI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. BaeI – 250 units
A restriction endonuclease that recognizes the sequence _NNNNN^NNNNNNNNNNACNNNNGTAYCNNNNNNN_NNNNN^.
New England Biolabs, Inc. BlpI – 500 units
A restriction endonuclease that recognizes the sequence GC^TNA_GC.